List of scientific publications related to projects funded by the Belgian Charcot Foundation

Disseminating knowledge to shed a light on future options

It is imperative that the results of both basic and clinical research be published to inform the medical and neurological scientific community. Even if some results are negative, it is important to make them known in order to shed light on future options and to trigger creative discussions on the reasons for any failure. This is both necessary and an ethical imperative in the dissemination of knowledge and in the long march towards increasing the effectiveness of treatments for a disease that remains incurable to this day.

Prof. Dr Christian Sindic President

2015-2022

• 2015

Broux B., … Somers V., … Stinissen P., Hellings N. IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in Multiple Sclerosis. Journal of Immunology, 2015, 194:2099-2109

Deckx N., Wens I., … Cools N. Rapid exercise-induced mobilization of dendritic cells is potentially mediated by a Flt3L- and MMP-9-dependent process in Multiple Sclerosis. Mediators of inflammation, 2015, article ID158956

Goris A.,…Dubois B., Harbo HF. Genetics variants are major determinants of CSF antibody levels in multiple sclerosis. Brain, 2015,138:632-643

Hilven K., Patsoupolos NA., Dubois B., Goris A. Burden of risk variants correlates with phenotype of Multiple Sclerosis. Multiple Sclerosis Journal, 2015,21:1670-1680

Muls N., … Sindic CJM., van Pesch V. Regulation of Treg-associated CD39 in multiple sclerosis and effects of vcorticotherapy during relapse. Multiple Sclerosis Journal, 2015,21:1533-1545

Ravanidis S., Bogie JFJ., … Stinissen P., … Hellings N. Neuroinflammatory signals enhance the immunomodulatory and neuroprotective properties of multipotent adult progenitor cells. Stem cell Research & Therapy, 2015, 6:76 (17pp)

• 2016

Claes N., Fraussen J., … Hellings N., … Somers V. Age-associated B cells with proinflammatory characteristics are expanded in a proportion of Multiple Sclerosis Patients. Journal of Immunology, 2016, 197:4576-4583

Dooley J. … Dubois B., Liston A., Goris A. Immunologic profiles of MS treatments reveal shared early B cell alterations. Neurology, Neuroimmunology & Neuroinflammation, 2016, 3:e240

Fraussen J., Claes N.,…Stinissen P., … Somers V. B cells of multiple sclerosis induce autoreactive proinflammatory T cell responses. Clinical Immunology, 2016,173:124-132

Gerwien H.,  … Opdenakker G., … Sorokin L. Imaging matrix metalloproteinase activity in Multiple Sclerosis as a specific marker of leukocyte penetration of the blood-brain-barrier. Science Translational Medicine;2016, 8:364ra152

Gonsette R., Debouverie M., Sindic CJM., Ferré JC., Edan G. Pixantrone: a B cell depleting immunosuppressant for MS patients with active disease. Multiple Sclerosis, 2016, 22:817-821

Gounko NV., Martens E., Opdenakker G., Rybakin V. Thymocyte development in the absence of matrix metalloproteinase-9/gelatinase B. Nature Scientific Reports, 2016, 6:29852

Guglielmetti C., Le Blon D., … Ponsaerts P. IL-13 immune gene therapy prevents CNS inflammation and demyelination via alternative activation of Microglia and Macrophages. Glia, 2016,, 64:2181-2200

Le Blon D., … Berneman ZW., Ponsaerts P. Intracerebral transplantation of IL 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model. Journal of Neuroinflammation, 2016, 13:288

Lee WP., Willekens B., Cras P., …Berneman ZW., Cools N. Immunomodulatory effects of 1,25-dihydroxyvitamin D3 on dendritic cells promote induction of T cell hyporesponsiveness to Myelin-derived antigens. Journal of Immunology Research, 2016, article ID5392623

Opdenaker G. et al. Microbiomic and posttranslational modifications as preludes to autoimmune diseases. Trends Molecular Medicine, 2016, 22:746-757

Zielinska KA., … Opdenakker G., … Van den Steen PE. Endothelial response to Glucocorticoids in inflammatory diseases. Frontiers in Immunology, 2016, 7:article 592

• 2017

Bogie, JFJ, … Grajchen E., … Hellings N., … Vanmierlo T., Hendriks JJA. Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes. Nature communications/scientific reports, 2017, 7:44794

De Laere M., … Berneman ZW., Cools N. Increased transendothelial transport of CCL3 is insufficient to drive immune cell transmigration through the BBB under inflammatory conditions in vitro. Mediators of inflammation, 2017, article ID6752756

Muls N., …Sindic CJM., van Pesch V. IL-22, GM-CSF and IL-17 in peripheral CD4+ T cell subpopulations during multiple sclerosis relapses and remission. Impact of corticosteroid therapy. PLoS One, 2017, 12:e0173780

Ravanidis S., Bogie JFJ., … Hendriks JJA., Stinissen P., … Hellings N. Crosstalk with inflammatory macrophages shapes the regulatory properties of Multipotent Adult Progenitor Cells. Stem Cells International, 2017, article 2353240

• 2018

De Laere M., Berneman ZW., Cools N. To the brain and back: migratory paths of dendritic cells in Multiple Sclerosis. Journal of Neuropathology and Experimental Neurology, 2018, 77:178-192

De Laere M., Derdelinckx J., …Grajchen E., Hendriks JJA, Bogie JFJ. The physiology of foamy phagocytes in multiple sclerosis. Acta Neuropathologica Communications, 2018, 6:124

Hilven K., … Goris A., Dubois B. Genetic basis for relapse rate in Multiple Sclerosis: Association with LRP2 genetic variation. Multiple Sclerosis Journal, 2018, 24:1773-1775

Lagou V.,…  Van Horebeek L.,… Dubois B.,… Goris A. Genetic architecture of adaptive immune system identifies key immune regulators. Cell Reports, 2018, 25:798-810

Maatveva O., Bogie JFJ., Hendriks JJA., … Kleinewietfeld M. Western lifestyle and immunopathology of Multiple Sclerosis. Annals of New York Academy of Sciences, 2018,1417:71-86

Smets I., … Dubois B., … Goris A., Sawcer S. Multiple Sclerosis risk variants alter expression of co-stimulatory genes in B cells. Brain, 2018, 141:786-796

Taj SH., Le Blon D., …Quarta A.,…Ponsaerts P., Hoehn M. Targeted intracerebral delivery of the anti-inflammatory cytokine IL13 promotes alternative activation of both microglia and macrophages after stroke. Journal of Neuroinflammation, 2018,15:174

Ugarte-Berzal E., … Vandooren J.,… Opdenakker G. Gelatinase B/matrix metalloproteinase-9 is a phase specific effector molecule, independent from Fas, in experimental autoimmune encephalomyelitis. PLoS ONE, 2018,13:e0197944

Uyttenhove C., … Van Snick J., … van Pesch V. Prophylactic treatment against GM-CSF, but not IL-17, abolishes relapses in a chronic murine model of multiple sclerosis. European Journal of Immunology, 2018, 48:1883-1891

Vandooren J. … Opdenakker G. Neutrophils and activated macrophages control mucosal immunity by proteolytic cleavage of antileukoproteinase. Frontiers in Immunology, 2018, 9;article 1154

Voet S., … Lamkanfi M., … van Loo G.  A20 critically controls microglia activation and inhibits inflammasome-dependent neuroinflammation. Nature Communications, 2018, 9:2036

• 2019

Boon L., Ugarte-Berzal E.,…Vandooren J., …Opdenakker G. Propeptide glycosylation and galectin-3 binding decrease proteolytic activation of human proMMP-9/progelatinase B. FEBS Journal, 2019,286:930-945

Derdelinckx J…. Cools N. Clinical and immunological control of experimental autoimmune encephalomyelitis by tolerogenic dendritic cells loaded with MOG-encoding mRNA. Journal of Neuroinflammation, 2019, 16:167

Fraussen J., … Somers V. Phenotypic and immunoglobulin repertoire analysis indicate a common origin of IgD- CD27- double negative (DN) B cells in healthy individuals and multiple sclerosis patients. Journal of Immunology, 2019, 203:1650-1664

Hassannia B., Vandenabeele P., Vanden Berghe T. Targeting ferroptosis to iron out Cancer. Cancer cell, 2019, 35:10:1016/2019.04.002

Lloyd AF., … des Rieux A., … Miron VE. Central nervous system regeneration is driven by microglia necroptosis and repopulation. Nature Neurosciences, 2019, 22:1046-1052

Quarta A., Le Blon D.,…Ponsaerts P. Murine iPSC-derived microglia and macrophage cell culture models recapitulate distinct phenotypical and functional properties of classical and alternative neuro-immune polarization. Brain, Behavior and Immunity, 2019, 82:406-421

Rybakin V., … Vandooren J, … Opdenakker G. Gelatinase B/matrix metalloproteinase-9 and other neutrophil proteases switch off interleukin-2 activity.  Biochemical Journal, 2019, 476:2191-2208

Schepers M., Tiane A., … , Piccart E., …Hellings N., …Vanmierlo T. Targeting phosphodiesterases-Towards a tailor-made approach in Multiple Sclerosis Treatment. Frontiers in Immunology, 2019, 10:1727

Tiane A., … Hellings N., … Vanmierlo T. From OPC to oligodendrocyte: an epigenetic journey. Cells, 2019, 8:1236

Ugarte-Berzal E., Boon L., …Vandooren J., …Opdenakker G. MMP-9/Gelatinase B degrades immune complexes in Systemic Lupus Erythematosus. Frontiers in Immunology, 2019,10:508

Vanden Berghe T. and E. Hoste. Paving the way for precision medicine v2.0 in intensive care by profiling necroinflammation in biofluids. Cell Death & Differentiation, 2019, 10:1038

Van Horebeek L., Dubois B., Goris A. Somatic variants : new kids on the block in human immunogenetics. Trends in Genetics, 2019, 35:935-947

Van Horebeek L…. Dubois B., Goris A. A robust pipeline with high replication rate for detection of somatic variants in the adaptive immune system as a source of common genetic variation in autoimmune disease. Human Molecular Genetics, 2019, 28:1369-1380

Van Schependom J., …D’hooghe MB., D’haeseleer M., …De Tiège X., Goldman S., …Nagels G. Altered transient brain dynamics in multiple sclerosis: Treatment or pathology? Human Brain Mapping, 2019,40:4789-4800

Voet S., Srinivasan S., Lamkanfi M., van Loo G. Inflammasomes in neuroinflammatory and neurodegenerative diseases.  Embo Molecular Medicine, 2019, 11:e10248

Voet S., Prinz M., van Loo G. Microglia in CNS inflammation and Multiple Sclerosis pathology. Trends Molecular Medicine, 2019, 25:112-123

• 2020

Baeten P., Hellings N., Broux B. In vitro tailoring of regulatory T cells prior to cell therapy. Trends in Molecular Medicine, 2020, 26:1059-1060

Bogie JFJ., Grajchen E., … Broux B., Stinissen P., … Hendriks JJA. CNS delivery of anti CD52 antibodies modestly reduces disease severity in an animal model for Multiple Sclerosis. Therapeutic Advances in Chronic Disease, 2020, 11:1-12

Bogie JFJ., Grajchen E., … Hendriks JJA. Stearoyl-CoA desaturase-1 impairs the reparative properties of macrophages and microglia in the central nervous system. Journal of Experimental Medicine, 2020,217:e20191660

Bogie JFJ., Haidar M., Kooij G., Hendriks JJA. Fatty acid metabolism in the progression and resolution of CNS disorders. Advanced Drug Delivery Reviews, 2020, 159:198-213

Boon L., Ugarte-Berzal E., Vandooren J., Opdenakker G. Protease propeptide structures, mechanisms of activation, and functions. Critical Reviews in Biochemistry and Molecular Biology, 2020,55:11-165

Costers L., Van Schependom J., ….De Tiège X., Goldman S., D’haeseleer M., D’hooghe MB., …Nagels G. Spatiotemporal and spectral dynamics of multi-item working memory as revealed by the n-back task using MEG. Human Brain Mapping, 2020,41:2431-2446

Derdelinckx J. …Willekens B., Cras P., Benerman ZN., Cools N. HLA Class II genotype does not affect the myelin responsiveness of Multiple Sclerosis patients. Cells, 2020, 9:2703

Grajchen E.,… Hendricks JJA., Bogie JFJ. CD36-mediated uptake of myelin debris by macrophages and microglia  reduces neuroinflammation. Journal of Neuroinflammation, 2020, 17:224 (14pp)

Holgado A. …Afonina IS., Beyaert R. Single-chain soluble receptor fusion proteins as versatile cytokine inhibitors. Frontiers in Immunology, 2020, 11:1422 (11pp)

Houben E. ..Vanmierlo T.,..Opdenaker G,....Broux B.,…Hellings N. Oncostatin M-induced astrocytic tissue inhibitor of metalloproteinases-1 drives remyelination. Proceedings of the National Academy of Sciences, 2020, 117:5028-5038

Jacobs W., …Vanden Berghe T., Jorens PG. Fatal lymphocytic cardiac damage in coronavirus disease 2019 (COVD-19): autopsy reveals a ferroptosis signature. ESC Heart Failure, 2020, 7:3772-3781

Martens A., … Voet;s., …Lamkanfi M., … van Loo G. Two distinct ubiquitin-binding motifs in A20 mediate its anti-inflammatory and cell-protective activities. Nature Immunology, 2020,21:381-387

Oldoni E., … Van Horebeek L.,…  Dubois B., Goris A. CHIT1 at diagnosis reflects long-term Multiple Sclerosis Disease activity. Annals of Neurology, 2020, 87:633-645

Opdenakker G., El-Asrar AA., Van Damme J. Remnant epitopes generating autoimmunity: from model to useful paradigm. Trends in Immunology, 2020,41:367-278

Orefice NS., … Muccioli GG. miRNA profiles is altered in a modified EAE mouse model of multiple sclerosis featuring cortical lesions. eLife, 2020, 9:e56916

Perdaens O.,…  van Pesch V. CSF microRNAs discriminate MS activity and share similarity to other neuroinflammatory disorders. Neurology, Neuroimmunolology & Neuroinflammation, 2020, 7:e673

Quarta A., Berneman Z., Ponsaerts P. Neuroprotective modulation of microglia effector functions following priming with interleukin 4 and 13: current limitations in understanding their mode-of-action. Brain, Behavior and Immunity, 2020, 88:856-866

Roggeri A., … Tiane A., Hellings N., … Vanmierlo T. Sphingosine-1-phosphate receptor modulators and oligodendroglial cells: beyond immunomodulation. International Journal of Molecular sciences, 2020, 21:7537

Serifova X., Ugarte-Berzal E., Opdenakker G., Vandooren J. Homotrimeric MMP-9 is an active hitchhiker on alpha-2-macroglobulin partially escaping protease inhibition and internalization through LRP-1. Cellular and Molecular Life Sciences, 2020,77:3013-3026

Van Horebeek L., Goris A. Transcript-specific regulation in T-cells in Multiple Sclerosis susceptibility. European Journal of Human Genetics, 2020, 28:849-850

Vanmierlo T., van Horssen J., Hellings N., Broux B. Editorial: Neuro-immune connections to enable repair in CNS disorders. Frontiers in Immunology, 2020, 11:1425

Wiernicki B., … Vandenabeele P., … Vanden Berghe T. Excessive phospholipid peroxidation distinguishes ferroptosis from other cell death modes including pyroptosis. Cell Death and Disease, 2020, 11:922

• 2021

Cauwels A. et al. Targeting IFN activity to both B cells and plasmacytoid dendritic cells induces a robust tolerogenic response and protection against EAE. Nature.communications/scientific reports, 2021, 11:21575

Ciccone L., Vandooren J., Nencetti S., Orlandini E. Natural marine and terrestrial componds as modulators of Matrix Mettaloproteinases-2 (MMP-2) and MMP-9 in Alzheimers’disease. Pharmaceuticals, 2021,14:86

Costers L., Van Schependom J., … De Tiège X., Goldman S., D’haeseleer M., D’hooghe MB., … Nagels G. The role of hippocampal theta oscillations in working memory impairment in Multiple Sclerosis. Human Brain Mapping, 2021, 42:1376-1390

Garcia Corales A.V., Haidar M., Bogie J.F.J., Hendriks J.J.A. Fatty acid synthesis in glial cells of the CNS. International Journal of Molecular Sciences, 2021,22:8159

Gratpain, V., … Muccioli GG., van Pesch V., des Rieux A. Extracellular vesicles for the treatment of central nervous system diseases. Advanced Drug Delivery Reviews, 2021, 174:535-552

Hassannia B., Van Coillie S., Vanden Berghe T. Ferroptosis : biological rust of lipid membranes. Antioxidants & Redox Signaling, 2021, 35:487-509

Liston A., Humblet-Baron S., Duffy D., Goris A. Human immune diversity: from evolution to modernity. Nature Immunology, 2021, 22:1479-1489

Meena M., … De Laere M.,… Benerman Z., Cools N. Transmigration across a steady-state Blood-Brain-Barrier induces activation of circulating dendritic cells partly mediated by actin cytoskeletal reorganization. Membranes, 2021, 11:700

Oltean T., … Vanden Berghe T., … Vandenabeele P. Viral dosing of influenza A infection reveals involvement of RIPK3 and FADD, but not MLKL. Cell Death and Disease, 2021, 12:471

Peters M., … Hellings N., … Ethirajan A. PEGylating poly(p-phenylene vinylene)-based bioimaging nanoprobes. Journal of colloid and interface science, 2021, 581:566-575

Quarta A., Benerman ZW., Ponsaerts P. Functional consequences of a close encounter between microglia and brain-infiltrating monocytes during CNS pathology and repair. Journal of Leukocytes Biology, 2021, 110:89-106

Quarta A…Le Blon D.,…Ponsaerts P. Murine induced pluripotent stem cell-derived neuroimmune cell culture models emphasize opposite immune-effector functions of interleukine 13-primed microglia and macrophages in terms of neuroimmune toxicity. Glia, 2021, 69:326-345

Smets I., … Dubois B., Matthys P., Liston A., Goris A. Treatment-induced BAFF expression and B cell biology in Multiple Sclerosis. Frontiers in Immunology, 2021, 12:676619

Smets I., Goris A., … Dubois B. Quantitative MRI phenotypes capture biological heterogeneity in Multiple Sclerosis patients. Nature Communications/ Scientific report, 2021,11:1573

Tiane A. Schepers M.,… Hellings N., …Vanmierlo T. DNA methylation regulates the expression of the negative transcriptional regulators ID2 and ID4 during OPC differentiation. Cellular and Molecular Life Sciences, 2021, 78:6631-6644

Vandebergh M., … Dubois B., Goris A. Genetic variation in WNT9B increases relapse hazard in Multiple Sclerosis. Annals of Neurology, 2021, 89:884-894

Vandooren J. … Opdenakker G. Internal disulfide bonding and glycosylation of interleukin-7 protect against proteolytic inactivation by neutrophil metalloproteinases and serine proteases. Frontiers in Immunology, 2021, 12:article 701739

Vandooren J. and Itoh I. Alpha-2-macroglobulin in inflammation, immunity and infections. Frontiers in Immunology, 2021,12:803244

Van Schependom J., … D’hooghe MB., D’haeseleer M., … De Tiège X., Goldman S., …Nagels G. Increased brain atrophy and lesion load is associated with stronger lower alpha MEG power in multiple sclerosis patients. Neuroimage: Clinical, 2021, 30:102632

Wens I., Janssens I., Derdelinckx., Meena M., Willekens B., Cools N. Made to measure: patient-tailored treatment of Multiple Sclerosis using cell-based therapies. International Journal of Molecular Sciences, 2021, 22:7536

• 2022​

Baeten P., Van Zeebroeck L., Kleinewietfeld M., Hellings N., Broux B . Improving the efficacy of regulatory T cell therapy. Clinical Reviews in Allergy & Immunology, 2022,62:363-381

Beliën J., Goris A., Matthys P. Natural killer cells in Multiple Sclerosis: entering the stage. Frontiers in Immunology, 2022,13:869447

Brocke S., Epstein P.M., Nelson R., Vanmierlo T. Editorial: Cyclic nucleotide phosphodiesterases (PDEs) in immune regulation and inflammation. Frontiers in Pharmacology, 2022,13:950480

Goris A.,Vandebergh M.,McCauley JL.,Saarela J., Cotsapas C. Genetics of multiple sclerosis: lessons from polygenicity. Lancet Neurology,2022,21:830-842

Guns J., Vanherle S., Hendriks JJA., Bogie JFJ. Protein lipidation by palmitate controls macrophage function. Cells, 2022, 11:565

Haidar, M., Loix M., …Bogie JFJ., Hendriks, JJA. Targeting lipophagy in macrophages improves repair in Multiple Sclerosis. Autophagy, 2022,18:2697-2710

Hassanabadi NS., Broux B., Marinivic S., Gotthardt D. Innate lymphoid cells-Neglected players in Multiple Sclerosis. Frontiers in Immunology,2022,13:909275

Hermans D., Houben E., Baeten P., …Fraussen J.,…Hellings N., Broux B. Oncostatin M triggers brain inflammation by compromising blood-brain barrier integrity. Acta Neuropathologica, 2022,144:259-281

Hoeks C., Duran G.,  Hellings N., Broux B. When helpers go above and beyond development and characterization of cytotoxic CD4+ T cells. Frontiers in Immunology, 2022,13:951900

Janssens I., ..., Van den Bos J., ..., Berneman Z.N., Wens I., Cools N. Engineering of regulatory cells by means of mRNA electroporation in a GMP-compliant manner. Cytotherapy, 2022,24:659-672

Labrak Y., … Muccioli GG., des Rieux A. Impact of anti-PDGFR alpha antibody surface functionalization on LNC uptake by oligodendrocyte progenitor cells. International Journal of Pharmaceutics, 2022, 618:121623.

Meena M., …  Berneman Z., Watts R., Cools N. A microfluidic in vitro three-dimensional dynamic model of the Blood-Brain-Barrier to study the transmigration of immune cells. Brain Sciences, 2022,12:1293

Van Breedam E., Berneman ZW., … Vanden Berghe T., … Ponsaerts P. Luminescent human IPSC-derived neurospheroids enable modelling of neurotoxicity after oxygen-glucose deprivation. Neurotherapeutics, 2022, doi.org/10.1007/s13311

Van Broeckhoven J., …Ponsaerts P., et al. Macrophage-based delivery of interleukin-13 improves functional and histopathological outcomes following spinal cord injury. Journal of Neuroinflammation, 2022,19:102